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Category: Research Antibodies

Scientists expose gaps in protein detection tests and suggest solution:

A new study highlights the need to create a better method for validating antibodies, and describes a process that would allow laboratories to ensure the proper functioning of their own.

In the laboratory and in the clinic, antibodies are used to study proteins, biomolecules which are the result of the translation of the genetic information of an organism and which play a role in the structure, function and regulation of its tissues. and organs. Genetic mutations can lead to protein imbalance or dysfunction, which can lead to disease.

Using antibodies, scientists can determine where and how much of a given type of protein is in the cell. Because of their usefulness, many companies manufacture antibodies for sale to research laboratories and clinical laboratories.

However, studies have shown that many antibodies already on the market do not specifically detect the proteins they are supposed to target. This finding calls into question all the studies that have used it, and demonstrates why it is necessary to develop a precise and standardized method to assess the quality of antibodies.

Under the direction of Peter McPherson, a team from The Neuro (Montreal Neurological Institute and Hospital) decided to use a human protein as an example, both to confirm the problem of antibody validation and to demonstrate the effectiveness of its new validation process.

The team chose the protein product of the C9ORF72 gene , whose mutations are the main genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia.

After testing 16 antibodies supposed to specifically detect the C9ORF72 protein, she discovered that only one detected it precisely with immunofluorescence, a technique that stains proteins to make them visible under a microscope. Only two antibodies have shown convincing results using other techniques. Antibodies that met the validation criteria have not yet been used in scientific studies, while the others have been used many times.

These results call into question the studies which have used to detect C9ORF72 antibodies which do not recognize the protein or which wrongly detect other proteins. They also highlight the need to improve validation methods.

Science is currently facing a crisis: the results of numerous studies have proven to be impossible to reproduce, and the lack of effective antibodies contributes to the problem in cell biology. In an article published on October 15, 2019 in the open access journal eLife , the authors describe their validation method, which other laboratories can reproduce to verify the effectiveness of their antibodies.

TOOLS Easy Fast RT Kit – from our innovative manufacturer Biotools

Posted on July 27, 2020July 27, 2020 by Ramona Byrd

TOOLS Easy Fast RT Kit – the fast first-strand cDNA synthesis with high efficiency with efficient and quick removal of the genomic DNA (gDNA). Tools Easy Fast RT Kit The kit contains RT enzyme, RNase inhibitor, random primer, oligo-dT primer, dNTP mixture and reaction buffer. It takes only 15 minutes (42 ° C) to complete the…

Highly sensitive ELISA for UV-induced DNA damage

Posted on July 27, 2020July 27, 2020 by Ramona Byrd

Most of the UV-induced DNA damage are cyclobutane pyrimidine dimers (CPDs) and (6-4) photoproducts (6-4PPs), that are fashioned between neighboring pyrimidine nucleotides on the identical DNA strand. These helix-distorting DNA lesions are solely repaired in people by a nucleotide excision restore system. The High Sensitivity CPD / Cyclobutane Pyrimidine Dimer ELISA equipment ( CSR-NM-MA-Okay003 ) from Cosmo Bio is the one commercially…

Health, well-being and social relations in a changing neighbourhood: protocol for a prospective, multimethods study of the consequences of large structural changes in an ethnic diverse social housing area in Denmark.

Health, well-being and social relations in a changing neighbourhood: protocol for a prospective, multimethods study of the consequences of large structural changes in an ethnic diverse social housing area in Denmark.

Posted on May 20, 2020May 20, 2020 by Ramona Byrd

Residential areas represent an vital enviornment for well being, well-being and social relations. Structural interventions equivalent to demolition and area renewal have been used to scale back inequality in well being and well-being in deprived areas. However, the results of bigger structural interventions are inconclusive. In a longitudinal perspective, this study goals to analyse how large-scale…

TSH Elisa kits

Posted on February 17, 2020February 17, 2020 by Ramona Byrd

Thyroid stimulating hormone detection on serum or biofluds canbe achieved by test from following suppliers:

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Posted on February 14, 2020February 17, 2020 by Ramona Byrd

IgD’s role has long been a conundrum and remains incompletely understood. IgD is present in species ranging from fish to human (probably with the exception of birds), suggesting that IgD was preserved since its ancient roots, which suggests that IgD has significant immunological functions. It’s understood that IgD signals for B cells to be activated…

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Posted on February 14, 2020February 14, 2020 by Ramona Byrd

What’s Flow Cytometry? FACS: categorized cells according to flow cytometry information Up on departing the flow room, electro-magnets will form cells charge in to different vessels. When more information is required, Compounds labeled with fluorescent dyes and increased against highly specific cell surface antigens (e.g. clusters of distinction or c d markers) enables you to…

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Recent Posts

  • TOOLS Easy Fast RT Kit – from our innovative manufacturer Biotools
  • Highly sensitive ELISA for UV-induced DNA damage
  • Health, well-being and social relations in a changing neighbourhood: protocol for a prospective, multimethods study of the consequences of large structural changes in an ethnic diverse social housing area in Denmark.
  • Pediatric Surgical Research Output in Germany in the Last 30 Years – An Assessment and International Comparison of Three Dedicated Paediatric Surgical Journals.
  • Spontaneous ventilation versus mechanical ventilation during video-assisted thoracoscopic surgery for spontaneous pneumothorax: a study protocol for multicenter randomized controlled trial.

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Health Pediatric Surgical Research Output in Germany in the Last 30 Years - An Assessment and International Comparison of Three Dedicated Paediatric Surgical Journals. Spontaneous ventilation versus mechanical ventilation during video-assisted thoracoscopic surgery for spontaneous pneumothorax: a study protocol for multicenter randomized controlled trial. well-being and social relations in a changing neighbourhood: protocol for a prospective
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