Most of the UV-induced DNA damage are cyclobutane pyrimidine dimers (CPDs) and (6-4) photoproducts (6-4PPs), that are fashioned between neighboring pyrimidine nucleotides on the identical DNA strand. These helix-distorting DNA lesions are solely repaired in people by a nucleotide excision restore system.
The High Sensitivity CPD / Cyclobutane Pyrimidine Dimer ELISA equipment ( CSR-NM-MA-Okay003 ) from Cosmo Bio is the one commercially obtainable ELISA utilizing the anti-CPD clone TDM-2 and was developed for the extremely sensitive detection of CPDs in cultured cells or DNA purified from the pores and skin dermis.
The High Sensitivity (6-4) photoproducts (6-4PPs) ELISA Kit ( CSR-NM-MA-Okay002 ) is the one commercially obtainable ELISA that makes use of the anti-6-4PPs clone 64M-2 and for the extremely sensitive one Detection of 6-4PPs from cultured cells from the pores and skin dermis was optimized.
This ELISA acknowledges CPDs or 6-4PPs from dipyrimidines in all DNA sequence contexts (eg TT, TC, CT and CC). Therefore, the provision and comfort of this ELISA equipment will assist to grasp the molecular mechanisms of mobile responses to UV radiation and DNA damage with functions in lots of areas of analysis akin to most cancers analysis, photobiology, dermatology, ophthalmology, immunology and beauty science.
To higher examine molecular occasions within the atmosphere of UV-induced DNA damage and restore, Mori et al. beforehand developed and characterised monoclonal antibodies (mAb) particularly for CPDs and mAb particularly for 6-4PPs (1), whereas Matsunaga et al. mAb developed and characterised particularly for Dewar picture merchandise (DewarPPs) (2).
Three of those antibodies (CPDs: clone TDM-2 [ CAC-NM-DND-001 ]; 6-4PPs: clone 64M-2 [ CAC-NM-DND-002 ]; DewarPPs: clone DEM-1 [ CAC-NM-DND -003 ]) proceed to be broadly cited within the literature, usually for use in ELISA by a really helpful methodology. These antibodies allow the quantification of photoproducts in purified DNA, these from cultured cells or from the pores and skin dermis utilizing an enzyme-linked immunoadsorption check (ELISA) and the visualization and measurement of photoproducts within the DNA from cultivated cells or the pores and skin by the use of oblique immunofluorescence (IIF).
1) Mori, T., et al., Photochem. Photobiol. 54, 225-232 (1991).
2) Matsunaga, T., et al., Photochem. Photobiol. 54, 403-410 (1991).