facs

What’s Flow Cytometry?


FACS: categorized cells according to flow cytometry information
Up on departing the flow room, electro-magnets will form cells charge in to different vessels.


When more information is required, Compounds labeled with fluorescent dyes and increased against highly specific cell surface antigens (e.g. clusters of distinction or c d markers) enables you to help identify and interrogate certain subpopulations in just a bigger group.


At a stream cytometry experimentation, every cell which moves through the flow cytometer and can be discovered will probably be categorized as a different event.


These cells generate forwards – and – side-scatter signs.


Side scattered light moves from the lighting source in to the stream station, is refracted by cells at a way that’s out the light course. Lighting is seen by a detector that’s orthogonal to the light path that was .
Also, every sort of lighting that’s discovered by the flow cytometer (forward-scatter, side-scatter( and every wavelength of fluorescence emission) is likely to soon be assigned a unique distinctive channel. Every occasion will be plotted by flow cytometry data and can reflect the signal intensity of lighting.


Initially developed in the late 1965s, flow cytometry can be an extremely more very favorite analytical cell-biology technique which uses light to profile and count cells at a heterogeneous fluid mix. Flow cytometry can be a method for the reason that it helps a researcher to rapidly and collect data linked by the fluid mix comprising cells.


At a FACS experimentation:


FACS can be really just actually a derivative of flow cytometry which adds an exemplary level of functionality. Employing FACS a research could form a mixture of cells into inhabitants that are various.


As an instance, from the storyline above, a researcher could find a way to distinguish between granulocytes and lymphocytes with forwards and side-scattered light. But, three types of granulocytes (neutrophils, basophils, and eosinophils) are much the same in size and arrangement, offering them comparable light-scattering properties. In this example, virtue of these saying of a mark such as ELANE could labels neutrophils.


Side-scattered lighting is generally utilized to produce a decision about the granularity and complexity of the cellphone while inside the light course. Cells having a great number of sophistication, having a low-granularity and sophistication, can produce light, and also also a signal compared to tissues such as neutrophils.


A mock flow cytometry dotplot, plotting forwards versus side-scattered in the people of leukocytes. Their own identity marks people:

Glue cells have been passed via a narrow channel one at one time.
Several kinds of lighting employed in a flow cytometry test.

Data acquired by the detectors will be compiled and incorporated to create a thorough picture of this sample.


The lighting can be utilized to illuminate the tissues from the station.
User-defined parameters offer advice how cells needs to be sprinkled.

Learn about various kinds of lighting employed in the amounts in a flow cytometry experimentation.


By comparison, in a scatter plot, each event is represented as one point onto a scatterplot. Intensity of two distinct stations (or 3 distinct stations at a three-dimensnal storyline ) are represented together the respective axes. Events with intensities that are similar will bunch in precisely exactly the exact identical region on the scatterplot.
Though a few customs can be supported by forward and side-scatter profiles, tagging using a celltype specific markers always supplies increased certainty and resolution as profiling complex heterogeneous populations of cells.

Using highly specific antibodies labeled with fluorescent dyes, so a researcher may do FACS investigation and simultaneously collect information on, and also form of an example with way of a nearly unlimited amount of unique parameters.


Multiparametric Diagnosis


Depending on these parameters, the FACS machine utilizes an electrode to inflict a electric charge on every cellphone.
A string of detectors to find the sorts of light which can be refracted or discharged from cells.


Fluorescent lighting is produced by fluorescent molecules after excitation using a harmonious wavelength laser. Light can arise from Compounds which were used to tag a particular arrangement or even dyes or can arise from fluorescing substances phone.
These cells create a moderate signal intensity that is reduced and forward-scatter.


The conditions of flow cytometry and fluorescence-activated cell sorting (FACS) are most usually used interchangeably. There are gaps between the 2 techniques.


Flow cytometry data is typically reflected in just one of 2 manners: histograms, that quantify or compare merely one parameter, also dot-plots which compare two or three parameters simultaneously on two- or threedimensional scatter plot.


A histogram an average of plots the seriousness found in one station along one axis and also the variety of events discovered at the strength is at another axis. A numbers of events discovered in one intensity is going to probably likely soon be displayed like a surge in the histogram.


Abstract


Forward scattered light is refracted with means of a cellphone inside the flow station and continues together from the light path (i.e.. The very exact same way the light had been originally traveling). Scattered light can be utilized to recognize the exact particle measurement and is detected by a detector at the path.


Read more about fluorescence or visit 50000 facs approved radicals labeled with fluorescent dyes.


Flow cytometry can be used widely during the entire life span and biomedical sciences, also will be implemented in virtually any scenario in which a scientist should rapidly profile a massive populace of cells that are loose from liquid media. By way of instance, at flow can be utilized different, to identify, and describe cell sub types that were immune .


Exactly what does flow cytometry data appear to be?


Multiparametric Diagnosis
Forward-scatter, side-scatter, along with fluorescent data are accumulated, as in traditional flow cytometry.
At a flow cytometer
D Presumed debris, and really tiny items with low forward- and side- scatter.

Forward scattered light is most widely utilized to find the magnitude of this thing at the light trail. Scattered light will be produced by Bigger items than objects, and cells that are bigger will have a scatter sign that is more powerful

Exo-APC Exosome FACS stain

EXOFLOW810A-1 SBI 20 reactions 241.00 EUR

EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence

K416-100 Biovision 490.00 EUR

EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence

K418-100 Biovision 490.00 EUR

EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

K452-100 Biovision 490.00 EUR

EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

K453-100 Biovision 490.00 EUR

EZClick? Myristoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

K497-100 Biovision 490.00 EUR

EZClick? Global Protein Synthesis Assay Kit (FACS/Microscopy), Green Fluorescence

K459-100 Biovision 550.00 EUR

EZClick? Global RNA Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence

K462-100 Biovision 533.00 EUR

EZClick? Global Protein Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence

K715-100 Biovision 550.00 EUR

EZClick? Global Phospholipid Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence

K717-100 Biovision 512.00 EUR

EZClick? Global RNA Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence

K718-100 Biovision 533.00 EUR

EZClick? O-GalNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

K560-100 Biovision 490.00 EUR

EZClickTM Fucose (FucAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

K592-100 Biovision 490.00 EUR

EZClick? O-GlcNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

K714-100 Biovision 512.00 EUR

EZClick? EdU Cell Proliferation/DNA Synthesis Kit (FACS/Microscopy), Red Fluorescence

K946-100 Biovision 533.00 EUR

EZClick? Sialic Acid (ManAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

K441-100 Biovision 490.00 EUR
Products Not found.

Flow Cytometry Reagents

Flow Cytometry Fixation/Permeabilization Kit

23006 Biotium 50test 80.00 EUR

Exo-Flow Multifunctional Magnetic Stand

EXOFLOW700A-1 SBI 1 Kit 402.00 EUR

CaspSCREEN? Flow Cytometric Apoptosis Detection Kit

K200-100 Biovision 523.00 EUR

CaspSCREEN? Flow Cytometric Apoptosis Detection Kit

K200-25 Biovision 278.00 EUR

Mouse IgG (-ve control for flow cytometry)

20008-1-100 Alpha Diagnostics 100 tests 103.00 EUR

Rabbit IgG (-ve control for flow cytometry)

20009-1-100 Alpha Diagnostics 100 tests 103.00 EUR

Micro Self Masking Continuous Flow Through Cell

SQ074 BBI Biotech 1 UNIT 156.82 EUR

Blocking Buffer for Lateral Flow (PBS based)

abx090808-500ml Abbexa 500 ml 384.00 EUR

Cell Meter™ Flow Cytometric Calcium Assay Kit

36310 AAT Bioquest 100 Tests 480.00 EUR

MycoLight™ Flow Cytometric Live Bacteria Assay Kit

22407 AAT Bioquest 100 Tests 306.00 EUR

Goat IgG (-ve control for flow cytometry) (isotype control)

20011-100 Alpha Diagnostics 100 test 103.00 EUR

Cell Meter™ Intracellular NADH/NADPH Flow Cytometric Analysis Kit

15291 AAT Bioquest 100 Tests 480.00 EUR

ExoFlow-ONE EV Labeling Kit for Flow Cytometry (Ruby Red)

EXOF100A-1 SBI 25 reactions 477.00 EUR

ExoFlow-ONE EV Labeling Kit for Flow Cytometry (Emerald Green)

EXOF300A-1 SBI 25 reactions 477.00 EUR

ExoFlow-ONE EV Labeling Kit for Flow Cytometry (Sapphire Blue)

EXOF400A-1 SBI 25 reactions 477.00 EUR

ExoFlow-ONE EV Labeling Kit for Flow Cytometry (Citrine Yellow)

EXOF500A-1 SBI 25 reactions 477.00 EUR

Exo-Flow 2.0 CD63 Analysis Kit (for Serum or Plasma)

EXOFLOW2-100A-SP SBI 10 rxn 588.00 EUR

Exo-Flow 2.0 CD9 Analysis Kit (for Serum or Plasma)

EXOFLOW2-105A-SP SBI 10 rxn 588.00 EUR

Exo-Flow 2.0 CD81 Analysis Kit (for Serum or Plasma)

EXOFLOW2-110A-SP SBI 10 rxn 588.00 EUR

Exo-Flow 2.0 CD63 Analysis Kit (for Tissue Culture Media)

EXOFLOW2-200A-TC SBI 10 rxn 588.00 EUR

Exo-Flow 2.0 CD9 Analysis Kit (for Tissue Culture Media)

EXOFLOW2-205A-TC SBI 10 rxn 588.00 EUR

Exo-Flow 2.0 CD81 Analysis Kit (for Tissue Culture Media)

EXOFLOW2-210A-TC SBI 10 rxn 588.00 EUR

Cell Meter™ Intracellular GSH Assay Kit *Optimized for Flow Cytometry*

22810 AAT Bioquest 100 Tests 219.00 EUR

ExoFlow-ONE EV Labeling Kit for Flow Cytometry (Garnet Far Red)

EXOF200A-1 SBI 25 reactions 477.00 EUR

Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit *Optimized for Flow Cytometry*

16317 AAT Bioquest 100 Tests 350.00 EUR

Cell Meter™ NIR Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry*

22802 AAT Bioquest 100 Tests 219.00 EUR

Cell Meter™ Nuclear Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry*

22811 AAT Bioquest 100 Tests 219.00 EUR

Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry*

22831 AAT Bioquest 100 Tests 219.00 EUR

Cell Meter™ Fluorimetric Mitochondrial Superoxide Activity Assay Kit*Optimized for Flow Cytometry*

22970 AAT Bioquest 100 Tests 306.00 EUR

Exo-Flow 2.0 Tetraspanin Combo Kit (CD63, CD9 & CD81) - for Serum or Plasma

EXOFLOW2-150A-SP SBI 30 rxn 1545.00 EUR

Exo-Flow 2.0 Tetraspanin Combo Kit (CD63, CD9 & CD81) - for Tissue Culture Media

EXOFLOW2-250A-TC SBI 30 rxn 1545.00 EUR

Cell Meter™ Mitochondrion Membrane Potential Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*

22804 AAT Bioquest 100 Tests 219.00 EUR

Cell Meter™ Mitochondrion Membrane Potential Assay Kit *Red Fluorescence Optimized for Flow Cytometry*

22806 AAT Bioquest 100 tests 219.00 EUR

Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry*

22832 AAT Bioquest 100 Tests 219.00 EUR

Cell Meter™ APC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*

22837 AAT Bioquest 100 Tests 219.00 EUR

Cell Meter™ PE-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*

22838 AAT Bioquest 100 Tests 219.00 EUR

Cell Meter™ FITC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*

22839 AAT Bioquest 100 Tests 219.00 EUR

Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Optimized for Flow Cytometry*

22904 AAT Bioquest 100 Tests 306.00 EUR

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma

EXOFLOW2-BASICA-SP SBI 30 rxn 1001.00 EUR

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Tissue Culture Media

EXOFLOW2-BASICA-TC SBI 30 rxn 1001.00 EUR

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence Optimized for Flow Cytometry*

11505 AAT Bioquest 100 Tests 263.00 EUR

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence Optimized for Flow Cytometry*

11506 AAT Bioquest 100 Tests 263.00 EUR

Live or Dead™ Fixable Dead Cell Staining Kit *Red Fluorescence Optimized for Flow Cytometry*

22599 AAT Bioquest 200 Tests 219.00 EUR

Cell Meter™ JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays*

22801 AAT Bioquest 100 Tests 306.00 EUR

Cell Meter™ Generic Fluorimetric Caspase Activity Assay Kit *Green Fluorescence Optimized for Flow Cytometry*

22821 AAT Bioquest 100 Tests 219.00 EUR

Cell Meter™ Generic Fluorimetric Caspase Binding Assay Kit *Red Fluorescence Optimized for Flow Cytometry*

22822 AAT Bioquest 100 Tests 219.00 EUR

Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry*

22824 AAT Bioquest 100 Tests 219.00 EUR

Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*

22825 AAT Bioquest 100 Tests 219.00 EUR

Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Red Fluorescence Optimized for Flow Cytometry*

22826 AAT Bioquest 100 Tests 219.00 EUR

Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Green Fluorescence Optimized for Flow Cytometry*

22841 AAT Bioquest 100 Tests 219.00 EUR

Cell Meter™ Fluorimetric Fixed Cell Cycle Assay Kit *Red Fluorescence Optimized for Flow Cytometry*

22842 AAT Bioquest 100 Tests 219.00 EUR

Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Red Fluorescence Optimized for Flow Cytometry*

22860 AAT Bioquest 100 tests 306.00 EUR

Fluorescein isothiocyanate (FITC) labelled antibodies for FACS

Aleuria Aurantia Lectin (Aal)-FITC conjugate

AAL15-FITC Alpha Diagnostics 0.5 ml 225.00 EUR

Rabbit Anti-Salmonella IgG-FITC conjugate

SLM11-FITC Alpha Diagnostics 0.5 ml 347.00 EUR

Sambucus nigra Lectin (SNA)-FITC conjugate

SNA15-FITC Alpha Diagnostics 0.5 ml 286.00 EUR

Lens culinaris Lectin (LCA)-FITC conjugate

LCA15-FITC Alpha Diagnostics 0.5 ml 286.00 EUR

Concanavalin A Lectin (ConA)-FITC conjugate

CONA15-FITC Alpha Diagnostics 0.5 ml 286.00 EUR

Wheat germ agglutinin Lectin (WGA)-FITC conjugate

WGA15-FITC Alpha Diagnostics 0.5 ml 286.00 EUR

Recombinant (E.Coli) purified Protein G-FITC Conjugate

PRTG15-FITC Alpha Diagnostics 1 ml 202.00 EUR

Goat Anti-Mouse albumin IgG-FITC Conjugate

ALB11-FITC Alpha Diagnostics 100 ug 347.00 EUR

Goat Anti-Human albumin IgG-FITC Conjugate

ALBH13-FITC Alpha Diagnostics 100 ug 347.00 EUR

Goat Anti-Mouse IgG (Fc) IgG-FITC Conjugate

40151-FITC Alpha Diagnostics 1 ml 225.00 EUR

Ricinus communis (castor bean) Agglutinin (RCA120)-FITC conjugate

RCA15-FITC Alpha Diagnostics 0.5 ml 225.00 EUR

Rabbit Anti-Luciferase (Photobacterium fischerii) IgG-FITC Conjugate

LUCF12-FITC Alpha Diagnostics 500 ul 384.00 EUR

Monoclonal Anti-Adenovirus (many isotypes) IgG-FITC conjugate

ADV12-FITC Alpha Diagnostics 100 ul 469.00 EUR

Mouse Monoclonal Anti-Human IgG1 (Fc-region)-FITC conjugate

10121-FITC Alpha Diagnostics 0.5 ml 225.00 EUR

Mouse Monoclonal Anti-Human IgG2 (Fc-region)-FITC conjugate

10122-FITC Alpha Diagnostics 0.5 ml 225.00 EUR

Mouse Monoclonal Anti-Human IgG3 (Hinge-region)-FITC conjugate

10123-FITC Alpha Diagnostics 0.5 ml 286.00 EUR

Mouse Monoclonal Anti-Human IgG4 (Fc-region)-FITC conjugate

10124-FITC Alpha Diagnostics 0.5 ml 286.00 EUR

Goat Anti-Poliomyelitis Virus 1-3 IgG-FITC Conjugate

POLV13-FITC Alpha Diagnostics 0.5 ml 408.00 EUR

Goat Anti-Rubella virus (HPV77 strain) IgG-FITC conjugate

RUBL11-FITC Alpha Diagnostics 1 ml 347.00 EUR

Goat Anti-Adenovirus type 2, hexon IgG-FITC conjugate

ADV11-FITC Alpha Diagnostics 0.5 ml 408.00 EUR

Rabbit anti-Beta-Galactosidase (E. Coli) IgG-FITC conjugate

BGAL12-FITC Alpha Diagnostics 100 ul 347.00 EUR

Goat Anti-Human Respiratory Syncytial Virus (RSV) IgG-FITC conjugate

RSV12-FITC Alpha Diagnostics 0.5 ml 469.00 EUR

Goat Anti-Glutathione Transferase (GST, S. japonicum) IgG-FITC Conjugate

GST13-FITC Alpha Diagnostics 100 ul 347.00 EUR

Goat Anti-Hemeagglutinin fusion epitope (HA-tag) IgG-FITC conjugate

HA12-FITC Alpha Diagnostics 100 ug 347.00 EUR

Goat Anti-Poly-His (6xhis) (His-tag) IgG-FITC conjugate

HISP16-FITC Alpha Diagnostics 100 ul 347.00 EUR

Rabbit Anti-Bovine Serum Albumin (BSA) protein IgG-FITC Conjugate

ALBB14-FITC Alpha Diagnostics 0.5 ml 347.00 EUR

Monoclonal Anti-Human Beta Actin peptide (non-muscle) IgG-FITC Conjugate

ACTB12-FITC Alpha Diagnostics 100 ul 408.00 EUR

Mouse Monoclonal Anti-Actin (Pan, alpha, beta, gamma) IgG-FITC conjugate

ACTB24-FITC Alpha Diagnostics 100 ul 408.00 EUR

Rabbit Anti-Vaccinia virus (VACV/VV/small pox) IgG-FITC conjugate

VACV11-FITC Alpha Diagnostics 0.5 ml 408.00 EUR

Goat Anti-human C-Reactive Protein (CRP)-FITC Conjugate, aff pure

CRP11-FITC Alpha Diagnostics 100 ug 408.00 EUR

Goat Anti-Green Fluorescent Proteins (GFP, A. victoria) protein IgG-FITC conjugate

GFP11-FITC Alpha Diagnostics 100 ug 347.00 EUR

Monoclonal Anti-Green Fluorescent Proteins (GFP, A. victoria) protein IgG-FITC conjugate

GFP12-FITC Alpha Diagnostics 0.5 ml 408.00 EUR

F(ab')2 fragment, Goat Anti-Mouse IgG (Fab)2 IgG-FITC Conjugate

40155-FITC Alpha Diagnostics 0.5 ml 263.00 EUR

Mouse mono Anti-human CRP (C-Reactive Protein) (clone 1) IgG-FITC conjugate

CRP13-FITC Alpha Diagnostics 100 ug 347.00 EUR

Mouse monoclonal Anti-Rabies Virus IgG-FITC conjugate (DFA II mix of 2 clones)

RBV15-FITC Alpha Diagnostics 1 ml 286.00 EUR

Rabbit Anti-purified protein derivative (PPD and most proteins of M. tuberculosis) IgG-FITC conjugate

PPD11-FITC Alpha Diagnostics 100 ul 445.00 EUR

F(ab')2 fragment,Goat Anti-Mouse IgG (Fc) IgG-FITC Conjugate (adsorbed with bovine, horse, and human serum proteins)

40152-FITC Alpha Diagnostics 0.5 ml 250.00 EUR

LDV FITC

B5644-1 ApexBio 1 mg 431.00 EUR

FITC Antibody

1-CSB-PA00511I0Rb Cusabio
  • 317.00 EUR
  • 335.00 EUR
  • 100ug
  • 50ug

Geldanamycin- FITC

abx076806-1mg Abbexa 1 mg 467.00 EUR

mPEG-FITC

abx085335-035kDa100mg Abbexa 0.35 kDa; 100 mg 773.00 EUR

mPEG-FITC

abx085335-055kDa100mg Abbexa 0.55 kDa; 100 mg 773.00 EUR

mPEG-FITC

abx085335-075kDa100mg Abbexa 0.75 kDa; 100 mg 773.00 EUR

mPEG-FITC

abx085335-20kDa100mg Abbexa 20 kDa; 100 mg 940.00 EUR

mPEG-FITC

abx085335-30kDa100mg Abbexa 30 kDa; 100 mg 940.00 EUR

Streptavidin (FITC)

abx670348-1mg Abbexa 1 mg 398.00 EUR

Streptavidin (FITC)

abx670352-1mg Abbexa 1 mg 495.00 EUR

Antibody for Human JAK2 (pTyr570)

SPC-1000D-FITC Stressmarq 0.1ml 392.00 EUR

Antibody for Human JAK3 (pTyr980+pTyr981)

SPC-1001D-FITC Stressmarq 0.1ml 392.00 EUR

Antibody for Human MAP4K5 (pSer174)

SPC-1002D-FITC Stressmarq 0.1ml 392.00 EUR

Antibody for Human c-Kit (Ser821+ Tyr823)

SPC-1003D-FITC Stressmarq 0.1ml 392.00 EUR

Antibody for Human Ksr2 (pSer490)

SPC-1004D-FITC Stressmarq 0.1ml 392.00 EUR