Machine learning (ML) permits the evaluation of advanced and huge knowledge units and has the potential to enhance healthcare. The clinical microbiology laboratory, at the interface of clinical follow and diagnostics, is of particular curiosity for the growth of ML programs.This narrative overview goals to discover the present use of ML In clinical microbiology.References for…
Category: Jak Stat Signaling Pathway
The role of JAK-STAT signalling activation in hypertrophied ligamentum flavum.
Journal: World neurosurgeryFebruar/15/2020
Although previous studies have reported the expression of JAK1, STAT3 and phosphorylated p-STAT3 in hypertrophied ligamentum flavum (LF), the role of the JAK/STAT signalling pathway in hypertrophied LF has not been fully elucidated. The aim of this study was to identify the important JAK/STAT gene expression patterns of the three main receptors involved in this pathway, namely, IFNAR, IFN-γ and IL-6.The human LF specimens were obtained from 28 patients who underwent lumbar spine surgery for either degenerative lumbar canal stenosis (DLCS) (n=28) or lumbar disc herniation (LDH) (n=20). In this design, LDH patients served as the control group. Fibrosis degrees were showed Masson’s trichrome staining. The location and the expression profiling of JAK/STAT pathway were analysed using real-time polymerase chain reaction and western blotting. The thickness of the LF was measured with axial T1-weighted magnetic resonance imaging.The most severe fibrotic changes were on the dorsal side of the LF. IL-6 and IFN-I expression significantly increased on the dorsal side of the LF. While expression levels of IL-6R and IFNAR on the dural and dorsal side were significantly higher in the DLCS samples, IFN-γR and endothelial EGFR in LF samples showed a significant increase on only the dorsal side. JAK/STAT genes were significantly expressed particularly on the dorsal side.We think both IFNAR and IL-6 receptor-dependent JAK/STAT signalling pathways may be significant targets for drug development strategies for the treatment of ligamentum flavum hypertrophy.
Emre Delen; Oğuzhan Doğanlar; Özlem Delen; Zeynep Doğanlar; Cumhur Kılınçer